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  • Featured Product
  • KD/KO Validated

MAST1 Polyclonal antibody

MAST1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13305-1-AP

Synonyms

KIAA0973, MAST1, SAST, SAST170

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using MAST1 Polyclonal antibody (13305-1-AP)

    WB analysis of mouse brain using 13305-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13305-1-AP (MAST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using MAST1 Polyclonal antibody (13305-1-AP)

    IP experiment of mouse brain using 13305-1-AP

    IP result of anti-MAST1 (IP:13305-1-AP, 4ug; Detection:13305-1-AP 1:500) with mouse brain tissue lysate 4000ug.

Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13305-1-AP targets MAST1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAST1 fusion protein Ag4126
Full Name microtubule associated serine/threonine kinase 1
Calculated Molecular Weight 1570 aa, 171 kDa
Observed Molecular Weight 171 kDa
GenBank Accession NumberBC027985
Gene Symbol MAST1
Gene ID (NCBI) 22983
RRIDAB_10639043
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAST1(microtubule associated serine/threonine kinase 1) is also named as KIAA0973, SAST(syntrophin-associated serine/threonine-protein kinase), SAST170 and belongs to the family of microtubule-associated serine/threonine kinases, which has a unique structural feature with a serine/threonine kinase domain and a postsynaptic density protein-95/discs large/zona occludens-1 domain(PMID:12614677). This protein plays a role in a broad range of neural functions and it appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins.

Protocols

Product Specific Protocols
WB protocol for MAST1 antibody 13305-1-APDownload protocol
IP protocol for MAST1 antibody 13305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neuron

Mutations in MAST1 Cause Mega-Corpus-Callosum Syndrome with Cerebellar Hypoplasia and Cortical Malformations.

Authors - Ratna Tripathy
  • KO Validated
View Article
humanWB,IHC,IF,IP

Theranostics

CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells

Authors - Apoorvi Tyagi
View Article