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MAST3 Polyclonal antibody

MAST3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19507-1-AP

Synonyms

KIAA0561, MAST3

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using MAST3 Polyclonal antibody (19507-1-AP)

    WB analysis of Jurkat using 19507-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAST3 Polyclonal antibody (19507-1-AP)

    WB analysis using 19507-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAST3 Polyclonal antibody (19507-1-AP)

    WB analysis using 19507-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using MAST3 Polyclonal antibody (19507-1-AP)

    WB analysis of mouse lung using 19507-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MAST3 Polyclonal antibody (19507-1-AP)

    WB analysis of human brain using 19507-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MAST3 Polyclonal antibody (19507-1-AP)

    WB analysis of human brain using 19507-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19507-1-AP (MAST3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using MAST3 Polyclonal antibody (19507-1-AP)

    IP experiment of Jurkat using 19507-1-AP

    IP result of anti-MAST3 (IP:19507-1-AP, 4ug; Detection:19507-1-AP 1:500) with Jurkat cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MAST3 Polyclonal antibody (19507-1-AP)

    IHC staining of human kidney using 19507-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19507-1-AP (MAST3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MAST3 Polyclonal antibody (19507-1-AP)

    IHC staining of human kidney using 19507-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19507-1-AP (MAST3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MAST3 Polyclonal antibody (19507-1-AP)

    IF Staining of HepG2 using 19507-1-AP

    Immunofluorescent analysis of HepG2 cells, using MAST3 antibody 19507-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inJurkat cells, human brain tissue, mouse lung tissue, mouse brain tissue, rat brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19507-1-AP targets MAST3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name microtubule associated serine/threonine kinase 3
Calculated Molecular Weight 143 kDa
Observed Molecular Weight 144 kDa
GenBank Accession NumberNM_015016
Gene Symbol MAST3
Gene ID (NCBI) 23031
RRIDAB_10642565
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAST3, also named as KIAA0561, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. MAST3 catalyze the reaction: ATP + a protein = ADP + a phosphoprotein.

Protocols

Product Specific Protocols
WB protocol for MAST3 antibody 19507-1-APDownload protocol
IHC protocol for MAST3 antibody 19507-1-APDownload protocol
IF protocol for MAST3 antibody 19507-1-APDownload protocol
IP protocol for MAST3 antibody 19507-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols