MATR3 Polyclonal antibody
MATR3 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12202-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human lung cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | SH-SY5Y cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 11 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
Product Information
12202-2-AP targets MATR3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MATR3 fusion protein Ag2843 |
| Full Name | matrin 3 |
| Calculated Molecular Weight | 847 aa, 95 kDa |
| Observed Molecular Weight | 125 kDa |
| GenBank Accession Number | BC015031 |
| Gene Symbol | MATR3 |
| Gene ID (NCBI) | 9782 |
| RRID | AB_2281752 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MATR3, known as KIAA0723, is an 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa protein as a result of PMID: 28977530.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MATR3 antibody 12202-2-AP | Download protocol |
| IHC protocol for MATR3 antibody 12202-2-AP | Download protocol |
| IF protocol for MATR3 antibody 12202-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. | ||
Nat Commun An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis. | ||
Cell Rep SATB1 regulates 3D genome architecture in T cells by constraining chromatin interactions surrounding CTCF-binding sites | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tatyana (Verified Customer) (03-11-2023) | Good and specific nuclear staining. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h at RT incubation.
 |
FH Wael (Verified Customer) (08-22-2022) | around 50ug of total cell lysate Blocking: 5% skim milk in PBS Primary incubation: 1:1000 in 5% skim milk in PBS overnight, rotating 4C Washes : 3X 15mins of PBS(0.001% Tween) Secondary: 1:10000 in 5% skim milk in PBS, 1hr, RT Washes : 3X 15mins of PBS(0.001% Tween) Specific Band size around 100KD
 |
