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  • Featured Product
  • KD/KO Validated

MCM2 Polyclonal antibody

MCM2 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, RIP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10513-1-AP

Synonyms

BM28, CCNL1, cdc19, CDCL1, D3S3194, KIAA0030, MCM2, MITOTIN, Nuclear protein BM28

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MCM2 Polyclonal antibody (10513-1-AP)

    WB analysis using 10513-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 10513-1-AP (MCM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using MCM2 Polyclonal antibody (10513-1-AP)

    WB analysis of HEK-293 using 10513-1-AP

    WB result of MCM2 antibody (10513-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MCM2 Polyclonal antibody (10513-1-AP)

    IP experiment of HEK-293 using 10513-1-AP

    IP result of anti-MCM2 (IP:10513-1-AP, 4ug; Detection:10513-1-AP 1:800) with HEK-293 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using MCM2 Polyclonal antibody (10513-1-AP)

    IHC staining of human cervical cancer using 10513-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using MCM2 Polyclonal antibody (10513-1-AP)

    IHC staining of human cervical cancer using 10513-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10513-1-AP (MCM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MCM2 Polyclonal antibody (10513-1-AP)

    IHC staining of human tonsillitis using 10513-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10513-1-AP (MCM2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using MCM2 Polyclonal antibody (10513-1-AP)

    IHC staining of human tonsillitis using 10513-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10513-1-AP (MCM2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MCM2 Polyclonal antibody (10513-1-AP)

    IF Staining of HeLa using 10513-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM2 antibody (10513-1-AP) at dilution of 1:20 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of human gliomas tissue using MCM2 Polyclonal antibody (10513-1-AP)

    IF Staining of human gliomas using 10513-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using 10513-1-AP (MCM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of NIH/3T3 cells using MCM2 Polyclonal antibody (10513-1-AP)

    FC experiment of NIH/3T3 using 10513-1-AP

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 ug Anti-Human MCM2 (10513-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman cervical cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, human gliomas tissue
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10513-1-AP targets MCM2 in WB, IP, IF, FC, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCM2 fusion protein Ag0798
Full Name minichromosome maintenance complex component 2
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 116-125 kDa
GenBank Accession NumberBC007670
Gene Symbol MCM2
Gene ID (NCBI) 4171
RRIDAB_2142131
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.

Protocols

Product Specific Protocols
WB protocol for MCM2 antibody 10513-1-APDownload protocol
IHC protocol for MCM2 antibody 10513-1-APDownload protocol
IF protocol for MCM2 antibody 10513-1-APDownload protocol
IP protocol for MCM2 antibody 10513-1-APDownload protocol
FC protocol for MCM2 antibody 10513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Nature

CDC7-independent G1/S transition revealed by targeted protein degradation.

Authors - Jan M Suski
View Article
humanWB

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
View Article
humanIF

Nat Commun

Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes

Authors - Hana Polasek-Sedlackova
View Article
WB

Cancer Res

LncRNA AGPG confers endocrine resistance in breast cancer by promoting E2F1 activity

Authors - Shiyi Yu
View Article
humanWB,RIP

Int J Biol Sci

ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway

Authors - Shasha Li
View Article
humanWB

Biomaterials

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Authors - Hongyuan Song
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Mandi (Verified Customer) (03-02-2020)

Biotinylated secondary and Alex Fluor needed for decent signal vs non-specific staining

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Rat brain