• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

METTL6 Polyclonal antibody

METTL6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16527-1-AP

Synonyms

methyltransferase like 6, METTL6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using METTL6 Polyclonal antibody (16527-1-AP)

    WB analysis using 16527-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16527-1-AP (METTL6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using METTL6 Polyclonal antibody (16527-1-AP)

    IHC staining of human lung cancer using 16527-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16527-1-AP (METTL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using METTL6 Polyclonal antibody (16527-1-AP)

    IHC staining of human ovary using 16527-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using METTL6 Polyclonal antibody (16527-1-AP)

    IHC staining of human placenta using 16527-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using METTL6 Polyclonal antibody (16527-1-AP)

    IHC staining of human placenta using 16527-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using METTL6 Polyclonal antibody (16527-1-AP)

    IHC staining of human skin using 16527-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using METTL6 Polyclonal antibody (16527-1-AP)

    IHC staining of human brain using 16527-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using METTL6 Polyclonal antibody (16527-1-AP)

    IHC staining of human breast cancer using 16527-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16527-1-AP (METTL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human placenta tissue, human skin tissue, human brain tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16527-1-AP targets METTL6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL6 fusion protein Ag9768
Full Name methyltransferase like 6
Calculated Molecular Weight 255 aa, 30 kDa
Observed Molecular Weight 30-32 kDa
GenBank Accession NumberBC022400
Gene Symbol METTL6
Gene ID (NCBI) 131965
RRIDAB_2142190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for METTL6 antibody 16527-1-APDownload protocol
IHC protocol for METTL6 antibody 16527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Immunol

Identification and validation of a tyrosine metabolism-related prognostic prediction model and characterization of the tumor microenvironment infiltration in hepatocellular carcinoma

Authors - Yangying Zhou
View Article
humanWB

J Biol Chem

Three distinct 3-methylcytidine (m(3)C) methyltransferases modify tRNA and mRNA in mice and humans.

Authors - Luang Xu
  • KO Validated
View Article