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MICA Polyclonal antibody

MICA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12619-1-AP

Synonyms

MIC A, MICA, PERB11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MICA Polyclonal antibody (12619-1-AP)

    WB analysis using 12619-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12619-1-AP (MICA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MICA Polyclonal antibody (12619-1-AP)

    WB analysis of HeLa using 12619-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12619-1-AP (MICA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inK-562 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12619-1-AP targets MICA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MICA fusion protein Ag3298
Full Name MHC class I polypeptide-related sequence A
Calculated Molecular Weight 383 aa, 43 kDa
Observed Molecular Weight 43 kDa, 50-55 kDa
GenBank Accession NumberBC016929
Gene Symbol MICA
Gene ID (NCBI) 100507436
RRIDAB_10638612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531).

Protocols

Product Specific Protocols
WB protocol for MICA antibody 12619-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Inhibition of Checkpoint Kinase 1 (CHK1) Upregulates Interferon Regulatory Factor 1 (IRF1) to Promote Apoptosis and Activate Anti-Tumor Immunity via MICA in Hepatocellular Carcinoma (HCC)

Authors - Xicai Li
View Article
humanWB

Cancers (Basel)

MICA+ Tumor Cell Upregulated Macrophage-Secreted MMP9 via PROS1-AXL Axis to Induce Tumor Immune Escape in Advanced Hepatocellular Carcinoma (HCC)

Authors - Qiulin Wu
View Article