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MND1 Polyclonal antibody

MND1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 11636-1-AP

Synonyms

GAJ, MND1

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using MND1 Polyclonal antibody (11636-1-AP)

    WB analysis of human liver using 11636-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MND1 Polyclonal antibody (11636-1-AP)

    WB analysis using 11636-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MND1 Polyclonal antibody (11636-1-AP)

    WB analysis of HepG2 using 11636-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using MND1 Polyclonal antibody (11636-1-AP)

    WB analysis of mouse liver using 11636-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using MND1 Polyclonal antibody (11636-1-AP)

    WB analysis of HepG2 using 11636-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using MND1 Polyclonal antibody (11636-1-AP)

    WB analysis of human liver using 11636-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using MND1 Polyclonal antibody (11636-1-AP)

    WB analysis of human lung using 11636-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using MND1 Polyclonal antibody (11636-1-AP)

    WB analysis of human kidney using 11636-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using MND1 Polyclonal antibody (11636-1-AP)

    IP experiment of mouse liver using 11636-1-AP

    IP result of anti-MND1 (IP:11636-1-AP, 4ug; Detection:11636-1-AP 1:600) with mouse liver tissue lysate 6000ug.

Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, human lung tissue, human kidney tissue, human liver tissue, MCF-7 cells
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11636-1-AP targets MND1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MND1 fusion protein Ag2210
Full Name meiotic nuclear divisions 1 homolog (S. cerevisiae)
Calculated Molecular Weight 205 aa, 24 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC032142
Gene Symbol MND1
Gene ID (NCBI) 84057
RRIDAB_2266545
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MND1 antibody 11636-1-APDownload protocol
IP protocol for MND1 antibody 11636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Oncotarget

A study of meiomitosis and novel pathways of genomic instability in cutaneous T-cell lymphomas (CTCL).

Authors - Matthew Tsang
View Article
humanWB

Med Oncol

Meiotic nuclear divisions 1 promotes proliferation and metastasis in hepatocellular carcinoma and is a potential diagnostic and therapeutic target gene

Authors - Kai Tan
  • KD Validated
View Article