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MOCOS Polyclonal antibody

MOCOS Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16395-1-AP

Synonyms

HMCS, MCS, MoCo sulfurase, MOCOS, molybdenum cofactor sulfurase, MOS

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MOCOS Polyclonal antibody (16395-1-AP)

    WB analysis of HepG2 using 16395-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16395-1-AP (MOCOS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using MOCOS Polyclonal antibody (16395-1-AP)

    IP experiment of HepG2 using 16395-1-AP

    IP result of anti-MOCOS (IP:16395-1-AP, 4ug; Detection:16395-1-AP 1:300) with HepG2 cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using MOCOS Polyclonal antibody (16395-1-AP)

    IHC staining of human liver using 16395-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16395-1-AP (MOCOS antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using MOCOS Polyclonal antibody (16395-1-AP)

    IHC staining of human liver using 16395-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16395-1-AP (MOCOS antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MOCOS Polyclonal antibody (16395-1-AP)

    IF Staining of HepG2 using 16395-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16395-1-AP (MOCOS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16395-1-AP targets MOCOS in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MOCOS fusion protein Ag9487
Full Name molybdenum cofactor sulfurase
Calculated Molecular Weight 888 aa, 98 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC012079
Gene Symbol MOCOS
Gene ID (NCBI) 55034
RRIDAB_2297842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOCOS(Molybdenum cofactor sulfurase) is also named as MCS, MOS, hMCS and belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. It catalyzes the removal of elemental sulfur from cysteine to produce alanine. Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form. Defects in MOCOS are the cause of xanthinuria type 2 (XU2)(PMID:17368066).

Protocols

Product Specific Protocols
WB protocol for MOCOS antibody 16395-1-APDownload protocol
IHC protocol for MOCOS antibody 16395-1-APDownload protocol
IF protocol for MOCOS antibody 16395-1-APDownload protocol
IP protocol for MOCOS antibody 16395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols