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MPRIP Polyclonal antibody

MPRIP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20040-1-AP

Synonyms

KIAA0864, M RIP, MPRIP, MRIP, p116Rip, Rho interacting protein 3, RHOIP3, RIP3

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using MPRIP Polyclonal antibody (20040-1-AP)

    WB analysis of SH-SY5Y using 20040-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using MPRIP Polyclonal antibody (20040-1-AP)

    WB analysis of SKOV-3 using 20040-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using MPRIP Polyclonal antibody (20040-1-AP)

    WB analysis of A2780 using 20040-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SKOV-3 cells using MPRIP Polyclonal antibody (20040-1-AP)

    IP experiment of SKOV-3 using 20040-1-AP

    IP result of anti-MPRIP (IP:20040-1-AP, 4ug; Detection:20040-1-AP 1:500) with SKOV-3 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human ovary tissue using MPRIP Polyclonal antibody (20040-1-AP)

    IHC staining of human ovary using 20040-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20040-1-AP (MPRIP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using MPRIP Polyclonal antibody (20040-1-AP)

    IHC staining of human ovary using 20040-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20040-1-AP (MPRIP antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using MPRIP Polyclonal antibody (20040-1-AP)

    IHC staining of mouse ovary using 20040-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 20040-1-AP (MPRIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MPRIP Polyclonal antibody (20040-1-AP)

    IF Staining of HeLa using 20040-1-AP

    Immunofluorescent analysis of Hela cells, using MPRIP antibody 20040-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inSH-SY5Y cells, A2780 cells, SKOV-3 cells
Positive IP detected inSKOV-3 cells
Positive IHC detected inmouse ovary tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20040-1-AP targets MPRIP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myosin phosphatase Rho interacting protein
Calculated Molecular Weight 117 kDa
Observed Molecular Weight 116-140 kDa
GenBank Accession NumberNM_015134
Gene Symbol MPRIP
Gene ID (NCBI) 23164
RRIDAB_10694433
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MPRIP, also named as KIAA0864, MRIP, RHOIP3 and p116Rip, is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. MRPIP targets myosin phosphatase to the actin cytoskeleton. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. The antibody recognizes the C-term of isoform2 and isoform3 of MPRIP.

Protocols

Product Specific Protocols
WB protocol for MPRIP antibody 20040-1-APDownload protocol
IHC protocol for MPRIP antibody 20040-1-APDownload protocol
IF protocol for MPRIP antibody 20040-1-APDownload protocol
IP protocol for MPRIP antibody 20040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Glia

Ermin is a p116RIP -interacting protein promoting oligodendroglial differentiation and myelin maintenance.

Authors - Shan Wang
View Article
humanWB

RNA

DAP5 drives translation of specific mRNA targets with upstream ORFs in human embryonic stem cells

Authors - Maya David
View Article
humanWB

J Cancer

High Expression of lncRNA AFAP1-AS1 Promotes the Progression of Colon Cancer and Predicts Poor Prognosis.

Authors - Hao Bo
View Article