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MRPL10 Polyclonal antibody

MRPL10 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16652-1-AP

Synonyms

L10MT, L8mt, MRP L10, MRP L8, MRPL10, MRPL8, RPML8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPL10 Polyclonal antibody (16652-1-AP)

    WB analysis using 16652-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16652-1-AP (MRPL10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using MRPL10 Polyclonal antibody (16652-1-AP)

    IP experiment of mouse liver using 16652-1-AP

    IP result of anti-MRPL10 (IP:16652-1-AP, 4ug; Detection:16652-1-AP 1:300) with mouse liver tissue lysate 6000 ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using MRPL10 Polyclonal antibody (16652-1-AP)

    IHC staining of human testis using 16652-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16652-1-AP (MRPL10 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MRPL10 Polyclonal antibody (16652-1-AP)

    IHC staining of human testis using 16652-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16652-1-AP (MRPL10 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPL10 Polyclonal antibody (16652-1-AP)

    IF Staining of HepG2 using 16652-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MRPL10 antibody (16652-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HEK-293 mitochondrial
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16652-1-AP targets MRPL10 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL10 fusion protein Ag10087
Full Name mitochondrial ribosomal protein L10
Calculated Molecular Weight 261 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC052601
Gene Symbol MRPL10
Gene ID (NCBI) 124995
RRIDAB_2282109
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRPL10 antibody 16652-1-APDownload protocol
IHC protocol for MRPL10 antibody 16652-1-APDownload protocol
IF protocol for MRPL10 antibody 16652-1-APDownload protocol
IP protocol for MRPL10 antibody 16652-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

An in vitro system to silence mitochondrial gene expression.

Authors - Luis Daniel Cruz-Zaragoza
View Article