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MRPL54 Polyclonal antibody

MRPL54 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17683-1-AP

Synonyms

L54mt, MRP L54, MRPL54

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using MRPL54 Polyclonal antibody (17683-1-AP)

    WB analysis of mouse liver using 17683-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17683-1-AP (MRPL54 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MRPL54 Polyclonal antibody (17683-1-AP)

    IHC staining of human skin cancer using 17683-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using MRPL54 Polyclonal antibody (17683-1-AP)

    IHC staining of human skin cancer using 17683-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using MRPL54 Polyclonal antibody (17683-1-AP)

    IHC staining of human oesophagus using 17683-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using MRPL54 Polyclonal antibody (17683-1-AP)

    IHC staining of human oesophagus using 17683-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRPL54 Polyclonal antibody (17683-1-AP)

    IF Staining of HeLa using 17683-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17683-1-AP (MRPL54 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman skin cancer tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17683-1-AP is MRPL54 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL54 fusion protein Ag11782
Full Name mitochondrial ribosomal protein L54
Calculated Molecular Weight 138 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC065273
Gene Symbol MRPL54
Gene ID (NCBI) 116541
RRIDAB_2878429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRPL54 antibody 17683-1-APDownload protocol
IHC protocol for MRPL54 antibody 17683-1-APDownload protocol
IF protocol for MRPL54 antibody 17683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols