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RON, MST1R Polyclonal antibody

RON, MST1R Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11053-1-AP

Synonyms

CD136, CDw136, MSP receptor, MST1R, p185 Ron, Protein tyrosine kinase 8, PTK8, RON, RON, MST1R

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using RON, MST1R Polyclonal antibody (11053-1-AP)

    WB analysis of mouse lung using 11053-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11053-1-AP (RON, MST1R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RON, MST1R Polyclonal antibody (11053-1-AP)

    IHC staining of human lung cancer using 11053-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11053-1-AP (RON, MST1R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RON, MST1R Polyclonal antibody (11053-1-AP)

    IHC staining of human lung cancer using 11053-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11053-1-AP (RON, MST1R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11053-1-AP targets RON, MST1R in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RON, MST1R fusion protein Ag1537
Full Name macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
Calculated Molecular Weight 152 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBQ936677 NM_002447
Gene Symbol MST1R
Gene ID (NCBI) 4486
RRIDAB_874180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RON (also referred to as MST1R, or CD136) is a receptor tyrosine kinase that belongs to the MET proto-oncogene family. The precursor RON is produced as a glycosylated, single chain that is cleaved into a 185-kDa heterodimer of α (35 kDa) and β (150 kDa) subunits connected by a disulfide bond (PMID: 8062829). It is activated upon binding to its ligand, macrophage-stimulating protein (MSP, also known as MST1 and HGFL). RON signalling is essential for embryonic development and regulates many physiological processes including cell survival, migration and differentiation.

Protocols

Product Specific Protocols
WB protocol for RON, MST1R antibody 11053-1-APDownload protocol
IHC protocol for RON, MST1R antibody 11053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Ther Oncolytics

RUNX1/EGFR pathway contributes to STAT3 activation and tumor growth caused by hyperactivated mTORC1.

Authors - Wei Lin
View Article