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MTERF Polyclonal antibody

MTERF Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 16957-1-AP

Synonyms

MTERF, MTERF1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using MTERF Polyclonal antibody (16957-1-AP)

    WB analysis of HeLa using 16957-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16957-1-AP (MTERF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MTERF Polyclonal antibody (16957-1-AP)

    WB analysis of Jurkat using 16957-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16957-1-AP (MTERF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16957-1-AP targets MTERF in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTERF fusion protein Ag10640
Full Name mitochondrial transcription termination factor
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC000965
Gene Symbol MTERF
Gene ID (NCBI) 7978
RRIDAB_2235580
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial transcription termination factor (MTERF)also named as Mterf1 is a 399 amino acid protein, which localizes in the mitochondrion and belongs to the mTERF family. MTERF contains three leucine zippers that form a three-stranded coiled-coil that binds to DNA. It has been suggested that only the phosphorylated form of MTERF has transcription termination activity. MTERF plays a central role in the control of mitochondrial rRNA and mRNA synthesis. MTERFD1 is also thought to act as a mitochondrial transcription regulator and is expressed as two isoforms produced by alternative splicing.Using DNA affinity chromatography, a fraction called MTERF was found to be associated with foot-printing capacity, and this contained two polypeptides of 34 and 31 kDa. Moreover, termination-promoting activity appears to reside in the 34-kDa protein. (PMID:7681833).

Protocols

Product Specific Protocols
WB protocol for MTERF antibody 16957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Immunol

Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.

Authors - Jing Geng
View Article
mouseWB

Nat Commun

Mice lacking the mitochondrial exonuclease MGME1 accumulate mtDNA deletions without developing progeria.

Authors - Stanka Matic
View Article