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MTMR4 Polyclonal antibody

MTMR4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 13488-1-AP

Synonyms

FYVE DSP2, KIAA0647, MTMR4, myotubularin related protein 4, ZFYVE11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MTMR4 Polyclonal antibody (13488-1-AP)

    WB analysis using 13488-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13488-1-AP (MTMR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MTMR4 Polyclonal antibody (13488-1-AP)

    IF Staining of HepG2 using 13488-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MTMR4 antibody (13488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13488-1-AP targets MTMR4 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTMR4 fusion protein Ag4310
Full Name myotubularin related protein 4
Calculated Molecular Weight 1195 aa, 133 kDa
Observed Molecular Weight 133 kDa
GenBank Accession NumberBC035609
Gene Symbol MTMR4
Gene ID (NCBI) 9110
RRIDAB_2251008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myotubularin-related protein-4 (MTMR4), also named FYVE-DSP2, is a member of the myotubularin family of phosphoinositide phosphatases that selectively degrade the 3-position phosphate from the inositol ring of PtdIns(3)P and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PMID: 31543504). MTMR4 knockdown markedly suppressed the motility, fusion, and fission of PI(3)P- enriched structures, resulting in decreases in late endosomes, autophagosomes, and lysosomes, and enlargement of PI(3)P- enriched early and late endosomes(PMID: 29962048).

Protocols

Product Specific Protocols
WB protocol for MTMR4 antibody 13488-1-APDownload protocol
IF protocol for MTMR4 antibody 13488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biol Chem

PtdIns3P phosphatases MTMR3 and MTMR4 negatively regulate innate immune responses to DNA through modulating STING trafficking.

Authors - Dyaningtyas Dewi Pamungkas Putri
View Article