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MUC8 Polyclonal antibody

MUC8 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55489-1-AP

Synonyms

MUC8, mucin 8

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using MUC8 Polyclonal antibody (55489-1-AP)

    WB analysis of A549 using 55489-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 55489-1-AP (MUC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MUC8 Polyclonal antibody (55489-1-AP)

    WB analysis of HeLa using 55489-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55489-1-AP (MUC8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MUC8 Polyclonal antibody (55489-1-AP)

    IHC staining of human lung cancer using 55489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MUC8 Polyclonal antibody (55489-1-AP)

    IHC staining of human lung cancer using 55489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using MUC8 Polyclonal antibody (55489-1-AP)

    IHC staining of human lung using 55489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using MUC8 Polyclonal antibody (55489-1-AP)

    IHC staining of human lung using 55489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MUC8 Polyclonal antibody (55489-1-AP)

    IHC staining of human lung cancer using 55489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MUC8 Polyclonal antibody (55489-1-AP)

    IHC staining of human lung cancer using 55489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MUC8 Polyclonal antibody (55489-1-AP)

    IHC staining of human lung cancer using 55489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MUC8 Polyclonal antibody (55489-1-AP)

    IHC staining of human lung cancer using 55489-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 55489-1-AP (MUC8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using MUC8 Polyclonal antibody (55489-1-AP)

    IF Staining of A549 using 55489-1-AP

    Immunofluorescent analysis of A549 cells using 55489-1-AP (MUC8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55489-1-AP targets MUC8 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mucin 8
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33-38 kDa
GenBank Accession NumberU14383
Gene Symbol MUC8
Gene ID (NCBI) 4590
RRIDAB_11182275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mucin glycoproteins (Mucins) are major constituents of the glycocalyx that covers mucosal epithelium. MUC8 is one of the major mucins in the ethmoid mucosa and is upregulated by chronic inflammation. TNFa, IL-1b and a combination of both can significantly increase Mucin 8 mRNA levels, suggesting that a mixture of inflammatory mediators can synergistically increase secretion of mucin in human nasal epithelium. This antibody is specific to MUC8.

Protocols

Product Specific Protocols
WB protocol for MUC8 antibody 55489-1-APDownload protocol
IHC protocol for MUC8 antibody 55489-1-APDownload protocol
IF protocol for MUC8 antibody 55489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Diagnostics (Basel)

The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?

Authors - Martin Schicht
View Article