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MURC Polyclonal antibody

MURC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55464-1-AP

Synonyms

cavin‐4, MURC

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse heart tissue using MURC Polyclonal antibody (55464-1-AP)

    WB analysis of mouse heart using 55464-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 55464-1-AP (MURC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MURC Polyclonal antibody (55464-1-AP)

    IHC staining of mouse heart using 55464-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55464-1-AP (MURC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MURC Polyclonal antibody (55464-1-AP)

    IHC staining of mouse heart using 55464-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55464-1-AP (MURC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

55464-1-AP targets MURC in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name muscle-related coiled-coil protein
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 48-53 kDa
GenBank Accession NumberNM_001018116
Gene Symbol MURC
Gene ID (NCBI) 347273
RRIDAB_2881343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MURC antibody 55464-1-APDownload protocol
IHC protocol for MURC antibody 55464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nutrients

Dietary α-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA Protection.

Authors - Jake S Russell
View Article