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MYLIP Polyclonal antibody

MYLIP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15455-1-AP

Synonyms

BZF1, Idol, MIR, MYLIP, PP5242

Validation Data Gallery

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  • Western Blot (WB) analysis of human cerebellum tissue using MYLIP Polyclonal antibody (15455-1-AP)

    WB analysis of human cerebellum using 15455-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 15455-1-AP (MYLIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of CHO cells using MYLIP Polyclonal antibody (15455-1-AP)

    WB analysis of CHO using 15455-1-AP

    CHO cells were subjected to SDS PAGE followed by western blot with 15455-1-AP (MYLIP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human testis using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human testis using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human skin using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human skin using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human brain using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human brain using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human liver using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human liver using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human spleen using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human spleen using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human lung using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human lung using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human ovary using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human ovary using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human heart using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human heart using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human kidney using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human kidney using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human placenta using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MYLIP Polyclonal antibody (15455-1-AP)

    IHC staining of human placenta using 15455-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 15455-1-AP (MYLIP antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman cerebellum tissue, CHO cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

15455-1-AP targets MYLIP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.

Tested Reactivity human, mouse, rat, hamster
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYLIP fusion protein Ag7720
Full Name myosin regulatory light chain interacting protein
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 46-50 kDa
GenBank Accession NumberBC002860
Gene Symbol MYLIP
Gene ID (NCBI) 29116
RRIDAB_2235610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MYLIP antibody 15455-1-APDownload protocol
IHC protocol for MYLIP antibody 15455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Elife

SEC24A deficiency lowers plasma cholesterol through reduced PCSK9 secretion.

Authors - Chen Xiao-Wei XW
View Article
mouseWB

Lett Appl Microbiol

Xinnaokang improves cecal microbiota and lipid metabolism to target atherosclerosis.

Authors - R Yang
View Article