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MYLK2 Polyclonal antibody

MYLK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21173-1-AP

Synonyms

KMLC, MLCK, MLCK2, MYLK2, myosin light chain kinase 2, skMLCK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYLK2 Polyclonal antibody (21173-1-AP)

    WB analysis using 21173-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MYLK2 Polyclonal antibody (21173-1-AP)

    WB analysis using 21173-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using MYLK2 Polyclonal antibody (21173-1-AP)

    WB analysis of human skeletal muscle using 21173-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MYLK2 Polyclonal antibody (21173-1-AP)

    WB analysis of Jurkat using 21173-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21173-1-AP (MYLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYLK2 Polyclonal antibody (21173-1-AP)

    IHC staining of mouse skeletal muscle using 21173-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21173-1-AP (MYLK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYLK2 Polyclonal antibody (21173-1-AP)

    IHC staining of mouse skeletal muscle using 21173-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21173-1-AP (MYLK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human skeletal muscle tissue, Jurkat cells, A549 cells, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

21173-1-AP targets MYLK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYLK2 fusion protein Ag15424
Full Name myosin light chain kinase 2
Calculated Molecular Weight 596 aa, 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC069627
Gene Symbol MYLK2
Gene ID (NCBI) 85366
RRIDAB_10733123
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYLK2, also named as MLCK2, belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. MYLK2 catalyze the reaction: ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate. MYLK2 is implicated in the level of global muscle contraction and cardiac function. MYLK2 phosphorylates a specific serine in the N-terminus of a myosin light chain. Mutation of MYLK2 will cause the cardiomyopathy familial hypertrophic (CMH).

Protocols

Product Specific Protocols
WB protocol for MYLK2 antibody 21173-1-APDownload protocol
IHC protocol for MYLK2 antibody 21173-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury.

Authors - Anteneh Getachew
View Article
humanWB

Microbiol Spectr

Berberine Depresses Inflammation and Adjusts Smooth Muscle to Ameliorate Ulcerative Colitis of Cats by Regulating Gut Microbiota

Authors - Xueying Li
View Article