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MYO1D Polyclonal antibody

MYO1D Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14028-1-AP

Synonyms

KIAA0727, MYO1D, myosin ID, myr4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse lung tissue using MYO1D Polyclonal antibody (14028-1-AP)

    WB analysis of mouse lung using 14028-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 14028-1-AP (MYO1D antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MYO1D Polyclonal antibody (14028-1-AP)

    WB analysis of mouse brain using 14028-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14028-1-AP (MYO1D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using MYO1D Polyclonal antibody (14028-1-AP)

    IP experiment of mouse lung using 14028-1-AP

    IP result of anti-MYO1D (IP:14028-1-AP, 4ug; Detection:14028-1-AP 1:300) with mouse lung tissue lysate 4000ug.

Tested Applications

Positive WB detected inmouse lung tissue
Positive IP detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14028-1-AP targets MYO1D in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYO1D fusion protein Ag5176
Full Name myosin ID
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC030602
Gene Symbol MYO1D
Gene ID (NCBI) 4642
RRIDAB_2266818
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MYO1D antibody 14028-1-APDownload protocol
IP protocol for MYO1D antibody 14028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Transl Oncol

Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.

Authors - Xinlu Liu
View Article
humanIHC

Cytoskeleton (Hoboken)

Myosin VI is required for maintenance of brush border structure, composition, and membrane trafficking functions in the intestinal epithelial cell.

Authors - Hegan Peter S PS
View Article