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  • Featured Product
  • KD/KO Validated

NAGK Polyclonal antibody

NAGK Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15051-1-AP

Synonyms

GlcNAc kinase, GNK, HSA242910, N acetyl D glucosamine kinase, N acetylglucosamine kinase, NAGK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NAGK Polyclonal antibody (15051-1-AP)

    WB analysis using 15051-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15051-1-AP (NAGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using NAGK Polyclonal antibody (15051-1-AP)

    WB analysis of mouse testis using 15051-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15051-1-AP (NAGK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NAGK Polyclonal antibody (15051-1-AP)

    IHC staining of human kidney using 15051-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15051-1-AP (NAGK antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NAGK Polyclonal antibody (15051-1-AP)

    IHC staining of human kidney using 15051-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15051-1-AP (NAGK antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NAGK Polyclonal antibody (15051-1-AP)

    IF Staining of HeLa using 15051-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15051-1-AP (NAGK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, mouse testis tissue, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15051-1-AP targets NAGK in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAGK fusion protein Ag7034
Full Name N-acetylglucosamine kinase
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC001029
Gene Symbol NAGK
Gene ID (NCBI) 55577
RRIDAB_2152368
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NAGK antibody 15051-1-APDownload protocol
IHC protocol for NAGK antibody 15051-1-APDownload protocol
IF protocol for NAGK antibody 15051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

Glutamine deprivation triggers NAGK-dependent hexosamine salvage.

Authors - Sydney Campbell
  • KD Validated
View Article