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NANS Polyclonal antibody

NANS Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15163-1-AP

Synonyms

N acetylneuraminate synthase, NANS, SAS, Sialic acid synthase

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NANS Polyclonal antibody (15163-1-AP)

    WB analysis of HeLa using 15163-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15163-1-AP (NANS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NANS Polyclonal antibody (15163-1-AP)

    WB analysis of human brain using 15163-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15163-1-AP (NANS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NANS Polyclonal antibody (15163-1-AP)

    WB analysis of HepG2 using 15163-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15163-1-AP (NANS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NANS Polyclonal antibody (15163-1-AP)

    IP experiment of HepG2 using 15163-1-AP

    IP result of anti-NANS (IP:15163-1-AP, 4ug; Detection:15163-1-AP 1:500) with HepG2 cells lysate 3300 ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NANS Polyclonal antibody (15163-1-AP)

    IHC staining of human ovary tumor using 15163-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15163-1-AP (NANS Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NANS Polyclonal antibody (15163-1-AP)

    IHC staining of human ovary tumor using 15163-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15163-1-AP (NANS Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NANS Polyclonal antibody (15163-1-AP)

    IHC staining of human stomach cancer using 15163-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15163-1-AP (NANS Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NANS Polyclonal antibody (15163-1-AP)

    IHC staining of human stomach cancer using 15163-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15163-1-AP (NANS Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NANS Polyclonal antibody (15163-1-AP)

    IHC staining of human pancreas cancer using 15163-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15163-1-AP (NANS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NANS Polyclonal antibody (15163-1-AP)

    IHC staining of human pancreas cancer using 15163-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15163-1-AP (NANS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary tumor tissue, human stomach cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

15163-1-AP targets NANS in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NANS fusion protein Ag7271
Full Name N-acetylneuraminic acid synthase
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC000008
Gene Symbol NANS
Gene ID (NCBI) 54187
RRIDAB_2150690
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The sialic acids are a family of 9-carbon 2-keto-3-deoxy sugars. They are frequently the terminal sugars on secreted and cell surface glycoproteins and glycolipids. Sialic acids participate in many important biologic recognition events. NANS can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.

Protocols

Product Specific Protocols
WB protocol for NANS antibody 15163-1-APDownload protocol
IHC protocol for NANS antibody 15163-1-APDownload protocol
IP protocol for NANS antibody 15163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Exp Cell Res

Reanalysis of single-cell data reveals macrophage subsets associated with the immunotherapy response and prognosis of patients with endometrial cancer

Authors - Qianhua Wu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

PK (Verified Customer) (03-22-2022)

  • Applications: Western Blot
  • Cell Tissue Type: LUNG Fibroblast
NANS Antibody Western Blot validation ( dilution) in LUNG Fibroblast (Cat no:15163-1-AP)