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NBL1 Polyclonal antibody

NBL1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 12069-1-AP

Synonyms

D1S1733E, DAN, DAN domain family member 1, DAND1, NB, NBL1, NO3, Protein N03, Zinc finger protein DAN

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using NBL1 Polyclonal antibody (12069-1-AP)

    WB analysis of mouse colon using 12069-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 12069-1-AP (NBL1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12069-1-AP targets NBL1 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NBL1 fusion protein Ag2706
Full Name neuroblastoma, suppression of tumorigenicity 1
Calculated Molecular Weight 180 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC012037
Gene Symbol NBL1
Gene ID (NCBI) 4681
RRIDAB_10638604
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NBL1 (neuroblastoma suppression of tumorigenicity 1), also named as DAN, is the founding member of the DAN subfamily. It was originally identified as a putative tumor suppressor gene in a transformed fibroblast rat model (PMID: 9516839), whereby it was significantly down-regulated in v-src-transformed rat fibroblasts. The MW of this protein is 19 kDa, and this antibody specially recognises the 19 kDa protein.

Protocols

Product Specific Protocols
WB protocol for NBL1 antibody 12069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols