• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

NDUFA3 Polyclonal antibody

NDUFA3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17257-1-AP

Synonyms

B9, CI B9, Complex I B9, NDUFA3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using NDUFA3 Polyclonal antibody (17257-1-AP)

    WB analysis of human brain using 17257-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17257-1-AP (NDUFA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NDUFA3 Polyclonal antibody (17257-1-AP)

    WB analysis of HeLa using 17257-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17257-1-AP (NDUFA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

17257-1-AP targets NDUFA3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFA3 fusion protein Ag10912
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3, 9kDa
Calculated Molecular Weight65aa,7 kDa; 84aa,9 kDa
Observed Molecular Weight 9 kDa
GenBank Accession NumberBC011021
Gene Symbol NDUFA3
Gene ID (NCBI) 4696
RRIDAB_2150631
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NDUFA3 antibody 17257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1

Authors - Lingxiao Tong
View Article
humanWB

J Biol Chem

Complex I mutations synergize to worsen the phenotypic expression of Leber's hereditary optic neuropathy.

Authors - Yanchun Ji
View Article
humanWB

Invest Ophthalmol Vis Sci

Assocation Between Leber's Hereditary Optic Neuropathy and MT-ND1 3460G>A Mutation-Induced Alterations in Mitochondrial Function, Apoptosis, and Mitophagy.

Authors - Juanjuan Zhang
View Article