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NDUFAF2 Polyclonal antibody

NDUFAF2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13891-1-AP

Synonyms

B17.2 like, B17.2L, mimitin, Mimitin, mitochondrial, MMTN, NDUFA12 like protein, NDUFA12L, NDUFAF2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using NDUFAF2 Polyclonal antibody (13891-1-AP)

    WB analysis of HepG2 using 13891-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13891-1-AP (NDUFAF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using NDUFAF2 Polyclonal antibody (13891-1-AP)

    IP experiment of SH-SY5Y using 13891-1-AP

    IP result of anti-NDUFAF2 (IP:13891-1-AP, 4ug; Detection:13891-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NDUFAF2 Polyclonal antibody (13891-1-AP)

    IHC staining of mouse skeletal muscle using 13891-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13891-1-AP (NDUFAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NDUFAF2 Polyclonal antibody (13891-1-AP)

    IHC staining of mouse skeletal muscle using 13891-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13891-1-AP (NDUFAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13891-1-AP targets NDUFAF2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFAF2 fusion protein Ag4853
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2
Calculated Molecular Weight 169 aa, 20 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC033965
Gene Symbol NDUFAF2
Gene ID (NCBI) 91942
RRIDAB_10597105
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mimitin, also named as MMTN, B17.2L, NDUFAF2 and NDUFA12L, is a small mitochondrial protein upregulated by proinflammatory cytokines at the transcriptional and protein levels. It is reported as a possible chaperone for assembly of mitochondrial complex I. The MW of Mimitin is 17-25 kDa.

Protocols

Product Specific Protocols
WB protocol for NDUFAF2 antibody 13891-1-APDownload protocol
IHC protocol for NDUFAF2 antibody 13891-1-APDownload protocol
IP protocol for NDUFAF2 antibody 13891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV.

Authors - Margherita Protasoni
View Article
humanWB

J Med Genet

Respiratory chain complex I deficiency due to NDUFA12 mutations as a new cause of Leigh syndrome.

Authors - Ostergaard Elsebet E
View Article
humanIP

Redox Rep

Ndufaf2, a protein in mitochondrial complex I, interacts in vivo with methionine sulfoxide reductases

Authors - Sujin Park
View Article