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NDUFB1 Polyclonal antibody

NDUFB1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16902-1-AP

Synonyms

CI MNLL, CI SGDH, Complex I MNLL, MNLL, NDUFB1

Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using NDUFB1 Polyclonal antibody (16902-1-AP)

    WB analysis of A375 using 16902-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 16902-1-AP (NDUFB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using NDUFB1 Polyclonal antibody (16902-1-AP)

    WB analysis of human colon using 16902-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 16902-1-AP (NDUFB1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using NDUFB1 Polyclonal antibody (16902-1-AP)

    WB analysis of mouse heart using 16902-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16902-1-AP (NDUFB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFB1 Polyclonal antibody (16902-1-AP)

    IHC staining of human kidney using 16902-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16902-1-AP (NDUFB1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFB1 Polyclonal antibody (16902-1-AP)

    IHC staining of human kidney using 16902-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16902-1-AP (NDUFB1 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inA375 cells, mouse heart tissue, human colon tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

16902-1-AP targets NDUFB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFB1 fusion protein Ag10443
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 1, 7kDa
Calculated Molecular Weight12 kDa, 7 kDa
Observed Molecular Weight 7 kDa
GenBank Accession NumberBC009691
Gene Symbol NDUFB1
Gene ID (NCBI) 4707
RRIDAB_2150787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NDUFB1 antibody 16902-1-APDownload protocol
IHC protocol for NDUFB1 antibody 16902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Complex I mutations synergize to worsen the phenotypic expression of Leber's hereditary optic neuropathy.

Authors - Yanchun Ji
View Article
WB

Int J Neurosci

Dysferlinopathy: Mitochondrial Abnormalities in Human Skeletal Muscle.

Authors - Fuchen Liu
View Article
humanWB

Cell Rep

A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.

Authors - Hezhi Fang
View Article