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NDUFB2 Polyclonal antibody

NDUFB2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17614-1-AP

Synonyms

AGGG, CI AGGG, Complex I AGGG, NDUFB2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFB2 Polyclonal antibody (17614-1-AP)

    WB analysis using 17614-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    WB analysis of human heart using 17614-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    WB analysis of human heart using 17614-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    WB analysis of mouse heart using 17614-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    WB analysis of rat heart using 17614-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    WB analysis of mouse spleen using 17614-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of mouse brain using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17614-1-AP (NDUFB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of mouse brain using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17614-1-AP (NDUFB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human brain using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human brain using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human ovary using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human ovary using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human heart using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human heart using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human kidney using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human kidney using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human skin using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human skin using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human liver using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using NDUFB2 Polyclonal antibody (17614-1-AP)

    IHC staining of human liver using 17614-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293T cells, mouse spleen tissue, human heart tissue, mouse heart tissue, rat heart tissue, HuH-7 cells, MCF-7 cells, mouse brain tissue, mouse kidney tissue
Positive IHC detected inmouse brain tissue, human heart tissue, human kidney tissue, human skin tissue, human liver tissue, human ovary tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17614-1-AP targets NDUFB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFB2 fusion protein Ag11789
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa
Calculated Molecular Weight 95 aa, 11 kDa
Observed Molecular Weight 8-12 kDa
GenBank Accession NumberBC063026
Gene Symbol NDUFB2
Gene ID (NCBI) 4708
RRIDAB_2150944
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. NDUFB2, also named as CI-AGGG, is a nuclear encoded subunit located in the hydrophobic protein fraction of complex I(PMID:9878551). It may have a role in the regulation of molecular changes during the global cardiac ischemia/reperfusion injury during cardiopulmonary bypass (CPB). The full length 12 kDa protein has a transit peptide with 33 amino acids.

Protocols

Product Specific Protocols
WB protocol for NDUFB2 antibody 17614-1-APDownload protocol
IHC protocol for NDUFB2 antibody 17614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols