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NEK9 Polyclonal antibody

NEK9 Polyclonal Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

IF, ELISA

Conjugate

Unconjugated

Cat no : 51057-2-AP

Synonyms

KIAA1995, Nek8, NEK9, NERCC, NERCC1, Nercc1 kinase, NimA related kinase 8, NimA related protein kinase 9

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of COS-7 cells using NEK9 Polyclonal antibody (51057-2-AP)

    IF Staining of COS-7 using 51057-2-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed COS-7 cells using 51057-2-AP (NEK9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IF detected inCOS-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51057-2-AP targets NEK9 in IF, ELISA applications and shows reactivity with human, monkey samples.

Tested Reactivity human, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEK9 fusion protein Ag1689
Full Name NIMA (never in mitosis gene a)- related kinase 9
Calculated Molecular Weight 107 kDa
GenBank Accession NumberBC009336
Gene Symbol NEK9
Gene ID (NCBI) 91754
RRIDAB_2881241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is independently identified as a Nek6- and Ran GTPase- binding protein under a different name, Nercc1 (PMID:14660563). Nek9, together with the highly similar Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. Confocal microscopy identified that Nek9 was localized to the spindle poles at the metaphase stages and associated with the midbody at anaphase or telophase stage in both meiotic oocytes and the first mitotic embyros (PMID: 23159858). The endogenous Nek9 (with predicted molecular mass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer (PMID:21454704).

Protocols

Product Specific Protocols
IF protocol for NEK9 antibody 51057-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols