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  • Featured Product
  • KD/KO Validated

NHP2 Polyclonal antibody

NHP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15128-1-AP

Synonyms

NHP2, NHP2P, NOLA2, snoRNP protein NHP2

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using NHP2 Polyclonal antibody (15128-1-AP)

    WB analysis of SH-SY5Y using 15128-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using NHP2 Polyclonal antibody (15128-1-AP)

    WB analysis of Caco-2 using 15128-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NHP2 Polyclonal antibody (15128-1-AP)

    WB analysis of HeLa using 15128-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NHP2 Polyclonal antibody (15128-1-AP)

    IP experiment of HepG2 using 15128-1-AP

    IP result of anti-NHP2 (IP:15128-1-AP, 4ug; Detection:15128-1-AP 1:500) with HepG2 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NHP2 Polyclonal antibody (15128-1-AP)

    IHC staining of human prostate cancer using 15128-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15128-1-AP (NHP2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NHP2 Polyclonal antibody (15128-1-AP)

    IHC staining of human prostate cancer using 15128-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15128-1-AP (NHP2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NHP2 Polyclonal antibody (15128-1-AP)

    IF Staining of HepG2 using 15128-1-AP

    Immunofluorescent analysis of HepG2 cells, using NHP2 antibody 15128-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inSH-SY5Y cells, Caco-2 cells, HeLa cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15128-1-AP targets NHP2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, yeast
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NHP2 fusion protein Ag7251
Full Name NHP2 ribonucleoprotein homolog (yeast)
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17-20 kDa
GenBank Accession NumberBC000009
Gene Symbol NHP2
Gene ID (NCBI) 55651
RRIDAB_2267290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NHP2 belongs to the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. It is required for ribosome biogenesis and telomere maintenance, and for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.

Protocols

Product Specific Protocols
WB protocol for NHP2 antibody 15128-1-APDownload protocol
IHC protocol for NHP2 antibody 15128-1-APDownload protocol
IF protocol for NHP2 antibody 15128-1-APDownload protocol
IP protocol for NHP2 antibody 15128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Structure of human telomerase holoenzyme with bound telomeric DNA.

Authors - George E Ghanim
View Article
humanWB

Nature

Cryo-EM structure of substrate-bound human telomerase holoenzyme.

Authors - Thi Hoang Duong Nguyen
View Article
humanWB,IF

EMBO J

NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.

Authors - Maya Raghunandan
  • KD Validated
View Article
human,yeastWB

EMBO Rep

Nhp2 is a reader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis.

Authors - Julia S P Mawer
View Article
mouseIHC

Biomedicines

Comparative Proteomic Analysis of tPVAT during Ang II Infusion.

Authors - Xiuying Liang
View Article
humanWB

Mol Cell Biol

SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization.

Authors - D E MacNeil
View Article