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NIPSNAP3A Polyclonal antibody

NIPSNAP3A Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10751-1-AP

Synonyms

HSPC299, NIPSNAP3A, NIPSNAP4, Protein NipSnap homolog 3A, Protein NipSnap homolog 4, TASSC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    WB analysis using 10751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    WB analysis of COLO 320 using 10751-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    WB analysis of HEK-293 using 10751-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    IP experiment of mouse brain using 10751-1-AP

    IP result of anti-NIPSNAP3A (IP:10751-1-AP, 4ug; Detection:10751-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    IHC staining of human colon cancer using 10751-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10751-1-AP (NIPSNAP3A Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    IHC staining of human colon cancer using 10751-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10751-1-AP (NIPSNAP3A Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using NIPSNAP3A Polyclonal antibody (10751-1-AP)

    IF Staining of HEK-293 using 10751-1-AP

    Immunofluorescent analysis of HEK-293 cells using 10751-1-AP (NIPSNAP3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, COLO 320 cells, HEK-293 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10751-1-AP targets NIPSNAP3A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NIPSNAP3A fusion protein Ag1143
Full Name nipsnap homolog 3A (C. elegans)
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-33 kDa
GenBank Accession NumberBC005935
Gene Symbol NIPSNAP3A
Gene ID (NCBI) 25934
RRIDAB_2298420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
IHC protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
IF protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
IP protocol for NIPSNAP3A antibody 10751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Kristina (Verified Customer) (01-20-2022)

I tried a few antibodies for NIPSNAP3A in the past and this one was the most precise with the best result so far.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: LUHMES
NIPSNAP3A Antibody Western Blot validation (1:1000 dilution) in LUHMES (Cat no:10751-1-AP)