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NO66/C14orf169 Polyclonal antibody

NO66/C14orf169 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP

Conjugate

Unconjugated

Cat no : 22842-1-AP

Synonyms

C14orf169, hsNO66, MAPJD, NO66, NO66/C14orf169, Nucleolar protein 66

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NO66/C14orf169 Polyclonal antibody (22842-1-AP)

    WB analysis using 22842-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22842-1-AP (NO66/C14orf169 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using NO66/C14orf169 Polyclonal antibody (22842-1-AP)

    WB analysis of Jurkat using 22842-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22842-1-AP (NO66/C14orf169 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using NO66/C14orf169 Polyclonal antibody (22842-1-AP)

    WB analysis of A431 using 22842-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 22842-1-AP (NO66/C14orf169 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using NO66/C14orf169 Polyclonal antibody (22842-1-AP)

    IP experiment of Jurkat using 22842-1-AP

    IP result of anti-NO66/C14orf169 (IP:22842-1-AP, 4ug; Detection:22842-1-AP 1:500) with Jurkat cells lysate 4000ug.

Tested Applications

Positive WB detected inA431 cells, Jurkat cells, NCCIT cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22842-1-AP is NO66/C14orf169 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NO66/C14orf169 fusion protein Ag18721
Full Name chromosome 14 open reading frame 169
Calculated Molecular Weight 641 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC011350
Gene Symbol C14orf169
Gene ID (NCBI) 79697
RRIDAB_2879175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NO66, also known as C14orf169 and MAPJD, is a JmjC domain-containing oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. NO66 is highly conserved during evolution and shows a dual localization pattern, present in both the nucleoplasm and nucleolus (PMID: 14742713). It has been reported that NO66 participates in regulation of Osx transcriptional activity and plays an important role in osteoblast differentiation through interaction with Osx (PMID: 19927124; 23620590). Through interaction with MYC protein, NO66 plays a role in pulmonary carcinogenesis (PMID: 17308053).

Protocols

Product Specific Protocols
WB protocol for NO66/C14orf169 antibody 22842-1-APDownload protocol
IP protocol for NO66/C14orf169 antibody 22842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols