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NOL12 Polyclonal antibody

NOL12 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15456-1-AP

Synonyms

NOL12, Nop25, nucleolar protein 12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOL12 Polyclonal antibody (15456-1-AP)

    WB analysis using 15456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NOL12 Polyclonal antibody (15456-1-AP)

    WB analysis of HeLa using 15456-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using NOL12 Polyclonal antibody (15456-1-AP)

    WB analysis of SH-SY5Y using 15456-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NOL12 Polyclonal antibody (15456-1-AP)

    WB analysis of HepG2 using 15456-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NOL12 Polyclonal antibody (15456-1-AP)

    IP experiment of HeLa using 15456-1-AP

    IP result of anti-NOL12 (IP:15456-1-AP, 4ug; Detection:15456-1-AP 1:300) with HeLa cells lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NOL12 Polyclonal antibody (15456-1-AP)

    IF Staining of HepG2 using 15456-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15456-1-AP (NOL12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, HepG2 cells, Raji cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15456-1-AP targets NOL12 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOL12 fusion protein Ag7723
Full Name nucleolar protein 12
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC002808
Gene Symbol NOL12
Gene ID (NCBI) 79159
RRIDAB_2282718
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NOL12 antibody 15456-1-APDownload protocol
IF protocol for NOL12 antibody 15456-1-APDownload protocol
IP protocol for NOL12 antibody 15456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Global landscape of HIV-human protein complexes.

Authors - Stefanie Jäger
View Article
human,ratIHC,WB,IF,CoIP

Cell Death Discov

Protein expression of nucleolar protein 12 in the retina and its implication in protection of retina from UV irradiation damage

Authors - Jingtao Liu
  • KD Validated
View Article