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NRAS-Specific Polyclonal antibody

NRAS-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18296-1-AP

Synonyms

ALPS4, GTPase NRas, HRAS1, N ras, NRAS, NRAS1, NRAS-Specific, Transforming protein N Ras

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NRAS-Specific Polyclonal antibody (18296-1-AP)

    WB analysis using 18296-1-AP

    Recombinant fusion protein Ag27444 (KRAS-2A), Ag21645(KRAS-2B), Ag29746 (NRAS), and Ag23265(HRAS) were subjected to SDS PAGE followed by western blot with 18296-1-AP (NRAS-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NRAS-Specific Polyclonal antibody (18296-1-AP)

    WB analysis using 18296-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18296-1-AP (NRAS-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using NRAS-Specific Polyclonal antibody (18296-1-AP)

    WB analysis of MCF-7 using 18296-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 18296-1-AP (NRAS-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of human colon using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of human colon using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of human malignant melanoma using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of human kidney using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of human kidney using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of human kidney using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of human kidney using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of human colon cancer using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of human colon cancer using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of rat colon using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of rat colon using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of mouse colon using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of mouse colon using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using NRAS-Specific Polyclonal antibody (18296-1-AP)

    IHC staining of human malignant melanoma using 18296-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA431 cells, MCF-7 cells
Positive IHC detected inhuman colon tissue, human kidney tissue, human colon cancer tissue, rat colon tissue, mouse colon tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18296-1-AP targets NRAS-Specific in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name neuroblastoma RAS viral (v-ras) oncogene homolog
Calculated Molecular Weight 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC005219
Gene Symbol NRAS
Gene ID (NCBI) 4893
RRIDAB_2918061
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML). The antibody is specific to NRAS, can't recognize HRAS and KRAS.

Protocols

Product Specific Protocols
WB protocol for NRAS-Specific antibody 18296-1-APDownload protocol
IHC protocol for NRAS-Specific antibody 18296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols