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NUFIP2 Polyclonal antibody

NUFIP2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17752-1-AP

Synonyms

182 FIP, 82 FIP, FIP 82, FMRP interacting protein 2, KIAA1321, NUFIP2, PIG1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NUFIP2 Polyclonal antibody (17752-1-AP)

    WB analysis of HeLa using 17752-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17752-1-AP (NUFIP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NUFIP2 Polyclonal antibody (17752-1-AP)

    IP experiment of HeLa using 17752-1-AP

    IP result of anti-NUFIP2 (IP:17752-1-AP, 4ug; Detection:17752-1-AP 1:500) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using NUFIP2 Polyclonal antibody (17752-1-AP)

    IHC staining of human cerebellum using 17752-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 17752-1-AP (NUFIP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using NUFIP2 Polyclonal antibody (17752-1-AP)

    IHC staining of human cerebellum using 17752-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 17752-1-AP (NUFIP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17752-1-AP is NUFIP2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUFIP2 fusion protein Ag12094
Full Name nuclear fragile X mental retardation protein interacting protein 2
Calculated Molecular Weight 695 aa, 76 kDa
Observed Molecular Weight 76-82 kDa
GenBank Accession NumberBC108307
Gene Symbol NUFIP2
Gene ID (NCBI) 57532
RRIDAB_2878433
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NUFIP2 antibody 17752-1-APDownload protocol
IHC protocol for NUFIP2 antibody 17752-1-APDownload protocol
IP protocol for NUFIP2 antibody 17752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

Authors - David W Sanders
View Article
mouseIHC

Proc Natl Acad Sci U S A

Succinyl-CoA ligase ADP-forming subunit beta promotes stress granule assembly to regulate redox and drive cancer metastasis

Authors - Austin C Boese
View Article
humanWB

J Cell Biol

Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control.

Authors - Jonathan Bizarro
View Article

Neuron

Increased G3BP2-Tau interaction in tauopathies is a natural defense against Tau aggregation

Authors - Congwei Wang
View Article