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NUP155 Monoclonal antibody

NUP155 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A7F2

Cat no : 66359-1-Ig

Synonyms

155 kDa nucleoporin, KIAA0791, N155, nucleoporin 155kDa, Nucleoporin Nup155, NUP155

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK293 cells using NUP155 Monoclonal antibody (66359-1-Ig)

    WB analysis of HEK293 cells using 66359-1-Ig

    WB result of NUP155 antibody (66359-1-Ig, 1: 6000) with si-Control and si-NUP155 transfected HEK293 cells.

  • Western Blot (WB) analysis of HeLa cells using NUP155 Monoclonal antibody (66359-1-Ig)

    WB analysis of HeLa using 66359-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66359-1-Ig (NUP155 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using NUP155 Monoclonal antibody (66359-1-Ig)

    WB analysis of ROS1728 using 66359-1-Ig

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 66359-1-Ig (NUP155 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUP155 Monoclonal antibody (66359-1-Ig)

    IHC staining of human breast cancer using 66359-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66359-1-Ig (NUP155 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUP155 Monoclonal antibody (66359-1-Ig)

    IHC staining of human breast cancer using 66359-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66359-1-Ig (NUP155 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK293 cells, HeLa cells, ROS1728 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66359-1-Ig targets NUP155 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivitymouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NUP155 fusion protein Ag3638
Full Name nucleoporin 155kDa
Calculated Molecular Weight 1391 aa, 155 kDa
Observed Molecular Weight 140-155 kDa
GenBank Accession NumberBC039257
Gene Symbol NUP155
Gene ID (NCBI) 9631
RRIDAB_2881738
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUP155 is a nucleoporin, the major component of the nuclear pore complex (NPC) that is essential for nuclear envelope biogenesis. Mutation of NUP155 has been linked to cardiovascular disease.

Protocols

Product Specific Protocols
WB protocol for NUP155 antibody 66359-1-IgDownload protocol
IHC protocol for NUP155 antibody 66359-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neuron

Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination.

Authors - Zhixiong Liu
View Article
ratWB

J Ethnopharmacol

Xinfuli granule alleviates metabolic remodeling through inhibition of endoplasmic reticulum stress and mitochondrial injury in heart failure

Authors - Yan-Ni Su
View Article