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  • Featured Product
  • KD/KO Validated

NUP50 Polyclonal antibody

NUP50 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20798-1-AP

Synonyms

50 kDa nucleoporin, nucleoporin 50kDa, Nucleoporin Nup50, NUP50

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using NUP50 Polyclonal antibody (20798-1-AP)

    WB analysis of Jurkat using 20798-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20798-1-AP (NUP50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NUP50 Polyclonal antibody (20798-1-AP)

    WB analysis of HEK-293 using 20798-1-AP

    WB result of NUP50 antibody (20798-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUP50 transfected HEK-293 cells.

  • Western Blot (WB) analysis of mouse brain tissue using NUP50 Polyclonal antibody (20798-1-AP)

    WB analysis of mouse brain using 20798-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20798-1-AP (NUP50 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUP50 Polyclonal antibody (20798-1-AP)

    IHC staining of human breast cancer using 20798-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUP50 Polyclonal antibody (20798-1-AP)

    IHC staining of human breast cancer using 20798-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using NUP50 Polyclonal antibody (20798-1-AP)

    IHC staining of human colon using 20798-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using NUP50 Polyclonal antibody (20798-1-AP)

    IHC staining of human colon using 20798-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20798-1-AP (NUP50 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20798-1-AP targets NUP50 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUP50 fusion protein Ag14710
Full Name nucleoporin 50kDa
Calculated Molecular Weight 468 aa, 50 kDa
Observed Molecular Weight 50 kDa, 65 kDa
GenBank Accession NumberBC028125
Gene Symbol NUP50
Gene ID (NCBI) 10762
RRIDAB_10696184
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L. This antibody recognizes both of two isoforms.

Protocols

Product Specific Protocols
WB protocol for NUP50 antibody 20798-1-APDownload protocol
IHC protocol for NUP50 antibody 20798-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogene

DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.

Authors - Bin Chen
View Article
ratWB

J Cell Sci

Nucleoporin 50 mediates Kcna4 transcription to regulate cardiac electrical activity.

Authors - Xueting Gao
  • KD Validated
View Article