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NUP54 Polyclonal antibody

NUP54 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 16232-1-AP

Synonyms

54 kDa nucleoporin, nucleoporin 54kDa, Nucleoporin p54, NUP54

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NUP54 Polyclonal antibody (16232-1-AP)

    WB analysis of HeLa using 16232-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using NUP54 Polyclonal antibody (16232-1-AP)

    WB analysis of mouse heart using 16232-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using NUP54 Polyclonal antibody (16232-1-AP)

    WB analysis of mouse liver using 16232-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using NUP54 Polyclonal antibody (16232-1-AP)

    WB analysis of K-562 using 16232-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using NUP54 Polyclonal antibody (16232-1-AP)

    WB analysis of A549 using 16232-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NUP54 Polyclonal antibody (16232-1-AP)

    WB analysis of HepG2 using 16232-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NUP54 Polyclonal antibody (16232-1-AP)

    IF Staining of HepG2 using 16232-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16232-1-AP (NUP54 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, mouse liver tissue, HepG2 cells, mouse heart tissue, A549 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 16232-1-AP is NUP54 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUP54 fusion protein Ag8882
Full Name nucleoporin 54kDa
Calculated Molecular Weight 507 aa, 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC012559
Gene Symbol NUP54
Gene ID (NCBI) 53371
RRIDAB_10644484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NUP54 antibody 16232-1-APDownload protocol
IF protocol for NUP54 antibody 16232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

Authors - Melissa Kane
View Article
humanWB

J Exp Clin Cancer Res

Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner.

Authors - Zhaoyu Qin
View Article
mouseWB

Cell Rep

Nucleoporin Seh1 maintains Schwann cell homeostasis by regulating genome stability and necroptosis

Authors - Mei Wu
View Article

Dev Cell

Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination

Authors - Li Li
View Article