• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

OSBPL10 Polyclonal antibody

OSBPL10 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15491-1-AP

Synonyms

ORP 10, ORP10, OSBP related protein 10, OSBP9, OSBPL10

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using OSBPL10 Polyclonal antibody (15491-1-AP)

    WB analysis of HepG2 using 15491-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15491-1-AP (OSBPL10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using OSBPL10 Polyclonal antibody (15491-1-AP)

    IP experiment of HeLa cells using 15491-1-AP

    IP result of anti-OSBPL10 (15491-1-AP for IP and Detection).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using OSBPL10 Polyclonal antibody (15491-1-AP)

    IHC staining of human colon cancer using 15491-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15491-1-AP (OSBPL10 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using OSBPL10 Polyclonal antibody (15491-1-AP)

    IHC staining of human colon cancer using 15491-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15491-1-AP (OSBPL10 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using OSBPL10 Polyclonal antibody (15491-1-AP)

    IF Staining of HepG2 using 15491-1-AP

    Immunofluorescent analysis of HepG2 cells, using OSBPL10 antibody 15491-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15491-1-AP targets OSBPL10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OSBPL10 fusion protein Ag7861
Full Name oxysterol binding protein-like 10
Calculated Molecular Weight 84 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC003168
Gene Symbol OSBPL10
Gene ID (NCBI) 114884
RRIDAB_2158356
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OSBPL10, also known as ORP10, is a member of the oxysterol-binding protein family. OSBPL10 localizes on microtubules and acts as a regulator of cellular lipid biosyntheses and apolipoprotein B-100. Genetic variation in OSBPL10 is associated with dyslipidmias and peripheral artery disease.

Protocols

Product Specific Protocols
WB protocol for OSBPL10 antibody 15491-1-APDownload protocol
IHC protocol for OSBPL10 antibody 15491-1-APDownload protocol
IF protocol for OSBPL10 antibody 15491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta

ORP10, a cholesterol binding protein associated with microtubules, regulates apolipoprotein B-100 secretion.

Authors - Nissil? Eija E
View Article
humanIF,WB

Nat Commun

Regulation of cellular cholesterol distribution via non-vesicular lipid transport at ER-Golgi contact sites

Authors - Tomoki Naito
View Article