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OTUD5 Polyclonal antibody

OTUD5 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 21002-1-AP

Synonyms

Deubiquitinating enzyme A, DUBA, OTU domain containing 5, OTUD5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using OTUD5 Polyclonal antibody (21002-1-AP)

    WB analysis using 21002-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 21002-1-AP (OTUD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using OTUD5 Polyclonal antibody (21002-1-AP)

    WB analysis using 21002-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21002-1-AP (OTUD5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using OTUD5 Polyclonal antibody (21002-1-AP)

    IF Staining of NIH/3T3 using 21002-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 21002-1-AP (OTUD5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293T cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells, C6 cells
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 21002-1-AP is OTUD5 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OTUD5 fusion protein Ag15190
Full Name OTU domain containing 5
Calculated Molecular Weight 571 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC028225
Gene Symbol OTUD5
Gene ID (NCBI) 55593
RRIDAB_2878788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for OTUD5 antibody 21002-1-APDownload protocol
IF protocol for OTUD5 antibody 21002-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression

Authors - Qiangqiang Liu
View Article