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  • Featured Product
  • KD/KO Validated

OXSR1 Polyclonal antibody

OXSR1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15611-1-AP

Synonyms

KIAA1101, OSR1, oxidative stress responsive 1, OXSR1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OXSR1 Polyclonal antibody (15611-1-AP)

    WB analysis using 15611-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15611-1-AP (OXSR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using OXSR1 Polyclonal antibody (15611-1-AP)

    WB analysis of HeLa using 15611-1-AP

    WB result of OXSR1 antibody (15611-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXSR1 transfected HeLa cells.

  • Western Blot (WB) analysis of human liver tissue using OXSR1 Polyclonal antibody (15611-1-AP)

    WB analysis of human liver using 15611-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15611-1-AP (OXSR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using OXSR1 Polyclonal antibody (15611-1-AP)

    WB analysis of human testis using 15611-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 15611-1-AP (OXSR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using OXSR1 Polyclonal antibody (15611-1-AP)

    IP experiment of HeLa using 15611-1-AP

    IP result of anti-OXSR1 (IP:15611-1-AP, 4ug; Detection:15611-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using OXSR1 Polyclonal antibody (15611-1-AP)

    IP experiment of HEK-293 using 15611-1-AP

    IP result of anti-OXSR1 (IP:15611-1-AP, 4ug; Detection:15611-1-AP 1:300) with HEK-293 cells lysate 2320 ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using OXSR1 Polyclonal antibody (15611-1-AP)

    IHC staining of human prostate cancer using 15611-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15611-1-AP (OXSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using OXSR1 Polyclonal antibody (15611-1-AP)

    IHC staining of human prostate cancer using 15611-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15611-1-AP (OXSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using OXSR1 Polyclonal antibody (15611-1-AP)

    IF Staining of MCF-7 using 15611-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using OXSR1 antibody (15611-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, human liver tissue, human testis tissue, HeLa cells, Jurkat cells, U-251 cells
Positive IP detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15611-1-AP targets OXSR1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OXSR1 fusion protein Ag8002
Full Name oxidative-stress responsive 1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC008726
Gene Symbol OXSR1
Gene ID (NCBI) 9943
RRIDAB_2299030
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Oxidative-stress responsive 1(OXSR1) is also named as KIAA1101, OSR1 and belongs to the STE Ser/Thr protein kinase family. It contains an N-terminal Ste20-like ser/thr kinase domain and 2 C-terminal regions, which has a putative caspase-3 cleavage site at the end. OXSR1's interaction with WNK1 is required for NKCC function, and it modulates the G protein sensitivity of PAK by phosphorylation of PAK1.Western blot analysis detected Oxsr1 at an apparent molecular mass of 58 kD in all mouse tissues examined except thymus. Cell fractionation and immunofluorescence analysis of HeLa cells showed that OXSR1 was distributed throughout the cell and OXSR1 could phosphorylate a test substrate and itself(PMID:14707132).

Protocols

Product Specific Protocols
WB protocol for OXSR1 antibody 15611-1-APDownload protocol
IHC protocol for OXSR1 antibody 15611-1-APDownload protocol
IF protocol for OXSR1 antibody 15611-1-APDownload protocol
IP protocol for OXSR1 antibody 15611-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Bioengineered

Upregulation of Oxidative stress-responsive 1(OXSR1) Predicts Poor Prognosis and Promotes Hepatocellular Carcinoma Progression.

Authors - Jianhui Chen
View Article
ratWB,IF

Adv Sci (Weinh)

Modulation of Cerebrospinal Fluid Dysregulation via a SPAK and OSR1 Targeted Framework Nucleic Acid in Hydrocephalus

Authors - Qiguang Wang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Jennifer (Verified Customer) (03-23-2022)

Image provided from optimisation plate for OXSR1 for rat brain lysates. Analysed using Simple Western Analysis (WES) by Protein Simple. Finalised antibody and protein concentration for samples produced a single band with no non-specific binding.

  • Applications: Other
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Rat and mouse brain lysate
OXSR1 Antibody Other validation (1:100 dilution) in Rat and mouse brain lysate (Cat no:15611-1-AP)