P2RY13 Polyclonal antibody
P2RY13 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
Cat no : 20335-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells |
Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IHC | See 1 publications below |
Product Information
The immunogen of 20335-1-AP is P2RY13 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | P2RY13 fusion protein Ag14108 |
Full Name | purinergic receptor P2Y, G-protein coupled, 13 |
Calculated Molecular Weight | 354 aa, 41 kDa |
Observed Molecular Weight | 45 kDa |
GenBank Accession Number | BC041116 |
Gene Symbol | P2RY13 |
Gene ID (NCBI) | 53829 |
RRID | AB_2878674 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for P2RY13 antibody 20335-1-AP | Download protocol |
IHC protocol for P2RY13 antibody 20335-1-AP | Download protocol |
FC protocol for P2RY13 antibody 20335-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Health Sci Rep P2RY13 is a prognostic biomarker and associated with immune infiltrates in renal clear cell carcinoma: A comprehensive bioinformatic study | ||
Nat Methods SEVtras delineates small extracellular vesicles at droplet resolution from single-cell transcriptomes |