• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

PAFAH1B3 Polyclonal antibody

PAFAH1B3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20564-1-AP

Synonyms

PAF AH 29 kDa subunit, PAF AH subunit gamma, PAFAH subunit gamma, PAFAH1B3, PAFAHG

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using PAFAH1B3 Polyclonal antibody (20564-1-AP)

    WB analysis of human brain using 20564-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PAFAH1B3 Polyclonal antibody (20564-1-AP)

    WB analysis using 20564-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PAFAH1B3 Polyclonal antibody (20564-1-AP)

    WB analysis of human brain using 20564-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using PAFAH1B3 Polyclonal antibody (20564-1-AP)

    IHC staining of human spleen using 20564-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PAFAH1B3 Polyclonal antibody (20564-1-AP)

    IHC staining of human spleen using 20564-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PAFAH1B3 Polyclonal antibody (20564-1-AP)

    IF Staining of SH-SY5Y using 20564-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20564-1-AP (PAFAH1B3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20564-1-AP targets PAFAH1B3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29kDa
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26-29 kDa
GenBank Accession NumberNM_002573
Gene Symbol PAFAH1B3
Gene ID (NCBI) 5050
RRIDAB_10693534
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAFAH1B3, also named as PAFAHG, belongs to the 'GDSL' lipolytic enzyme family and Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily. It inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. PAFAH1B3 plays an important role during the development of brain. The antibody is specific to PAFAH1B3.

Protocols

Product Specific Protocols
WB protocol for PAFAH1B3 antibody 20564-1-APDownload protocol
IHC protocol for PAFAH1B3 antibody 20564-1-APDownload protocol
IF protocol for PAFAH1B3 antibody 20564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Onco Targets Ther

Aberrant expression of PAFAH1B3 associates with poor prognosis and affects proliferation and aggressiveness in hypopharyngeal squamous cell carcinoma.

Authors - Jianing Xu
  • KD Validated
View Article
humanIHC,IF

Discov Oncol

Increased PAFAH1B3 was associated with poor prognosis and T-cell exhaustion microenvironment in hepatocellular carcinoma

Authors - Genhao Zhang
View Article
humanIHC

Discov Oncol

Prognostic clinical phenotypes associated with tumor stemness in the immune microenvironment of T-cell exhaustion for hepatocellular carcinoma

Authors - Genhao Zhang
View Article