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PAM Polyclonal antibody

PAM Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 26972-1-AP

Synonyms

PAL, PAM, Peptidylamidoglycolate lyase, PHM

Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using PAM Polyclonal antibody (26972-1-AP)

    WB analysis of U2OS using 26972-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 26972-1-AP (PAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using PAM Polyclonal antibody (26972-1-AP)

    IHC staining of human colon using 26972-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26972-1-AP (PAM Antibody) at dilution of 1:200 (under 10x lens).

Tested Applications

Positive WB detected inU2OS cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 26972-1-AP is PAM Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAM fusion protein Ag25719
Full Name peptidylglycine alpha-amidating monooxygenase
Calculated Molecular Weight 108 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC018127
Gene Symbol PAM
Gene ID (NCBI) 5066
RRIDAB_2880706
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PAM antibody 26972-1-APDownload protocol
IHC protocol for PAM antibody 26972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
hamsterWB

Comp Biochem Physiol B Biochem Mol Biol

Change on apoptosis, autophagy and mitochondria of the Harderian gland in Cricetulus barabensis during age.

Authors - Xiao-Tong Kong
View Article