PCDH24 Polyclonal antibody

PCDH24 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27103-1-AP

Synonyms

CDHR2, PC LKC, PCDH24, PCLKC, protocadherin 24, Protocadherin LKC



Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue, human liver tissue, human small intestine tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27103-1-AP is PCDH24 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCDH24 fusion protein Ag25840
Full Name protocadherin 24
Calculated Molecular Weight 1310 aa, 142 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC130282
Gene Symbol PCDH24
Gene ID (NCBI) 54825
RRIDAB_2880757
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protocadherin-24 (PCDH24, also known as CDHR2 and protocadherin LKC) was originally identified as a novel member of the human non-classical cadherin family. PCDH24 may play a role in cell-cell adhesion and contact inhibition in epithelial cells. PCDH24 expression is markedly reduced in cancers derived from liver and colon tissues, indicating that PCDH24 may play a role as a tumor suppressor protein (PMID: 23115611). The calculated molecular weight of PCDH24 is 142 kDa.

Protocols

Product Specific Protocols
WB protocol for PCDH24 antibody 27103-1-APDownload protocol
IHC protocol for PCDH24 antibody 27103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Transl Androl Urol

Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.

Authors - Xuechao Li