- Featured Product
- KD/KO Validated
PDSS2 Polyclonal antibody
PDSS2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13544-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | SGC-7901 cells, A375 cells |
| Positive IHC detected in | human kidney tissue, human liver cancer tissue, mouse liver tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IF | See 2 publications below |
Product Information
13544-1-AP targets PDSS2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PDSS2 fusion protein Ag4487 |
| Full Name | prenyl (decaprenyl) diphosphate synthase, subunit 2 |
| Calculated Molecular Weight | 399 aa, 44 kDa |
| Observed Molecular Weight | 27-30 kDa, 44-47 kDa |
| GenBank Accession Number | BC039906 |
| Gene Symbol | PDSS2 |
| Gene ID (NCBI) | 57107 |
| RRID | AB_10598311 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDSS2, also named as C6orf210, is required for the synthesis of coenzyme Q10 (CoQ10), which is synthesized in the mitochondrial inner membrane and plays a vital role in the mitochondrial respiratory chain, pyrimidine nucleoside biosynthesis, and modulation of apoptosis (PMID:25330808). An increasing body of evidence suggests that PDSS2 is a tumor suppressor gene in certain cancers, such as melanoma, gastric cancer and non-small cell lung cancer. Besides, PDSS2 could be used as a prognostic biomarker for HCC (PMID:25780306). PDSS2 has 2 isoforms with MW of 44 kDa and 27 kDa while 13544-1-AP antibody detects the bands around 27-30 kDa and 44-47 kDa in SDS-PAGE.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDSS2 antibody 13544-1-AP | Download protocol |
| IHC protocol for PDSS2 antibody 13544-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Mol Med β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice. | ||
Aging Cell Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging. | ||
Neurol Genet Novel Homozygous Variant in COQ7 in Siblings With Hereditary Motor Neuropathy | ||
Mol Genet Metab Rep A novel COQ7 mutation causing primarily neuromuscular pathology and its treatment options | ||
Antioxidants (Basel) Co-Enzyme Q10 Supplementation Rescues Cumulus Cells Dysfunction in a Maternal Aging Model. |
