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PGBD3 Polyclonal antibody

PGBD3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 17639-1-AP

Synonyms

PGBD3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PGBD3 Polyclonal antibody (17639-1-AP)

    WB analysis of HeLa using 17639-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17639-1-AP (PGBD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PGBD3 Polyclonal antibody (17639-1-AP)

    WB analysis of HeLa using 17639-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17639-1-AP (PGBD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PGBD3 Polyclonal antibody (17639-1-AP)

    IHC staining of human kidney using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PGBD3 Polyclonal antibody (17639-1-AP)

    IHC staining of human kidney using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PGBD3 Polyclonal antibody (17639-1-AP)

    IHC staining of human ovary using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PGBD3 Polyclonal antibody (17639-1-AP)

    IHC staining of human ovary using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PGBD3 Polyclonal antibody (17639-1-AP)

    IHC staining of human testis using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PGBD3 Polyclonal antibody (17639-1-AP)

    IHC staining of human testis using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PGBD3 Polyclonal antibody (17639-1-AP)

    IHC staining of human brain using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PGBD3 Polyclonal antibody (17639-1-AP)

    IHC staining of human brain using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PGBD3 Polyclonal antibody (17639-1-AP)

    IHC staining of human spleen using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PGBD3 Polyclonal antibody (17639-1-AP)

    IHC staining of human spleen using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using PGBD3 Polyclonal antibody (17639-1-AP)

    IHC staining of human lung using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using PGBD3 Polyclonal antibody (17639-1-AP)

    IHC staining of human lung using 17639-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17639-1-AP (PGBD3 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17639-1-AP targets PGBD3 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGBD3 fusion protein Ag11766
Full Name piggyBac transposable element derived 3
Calculated Molecular Weight 593 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC063690
Gene Symbol PGBD3
Gene ID (NCBI) 267004
RRIDAB_2262195
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PGBD3 antibody 17639-1-APDownload protocol
IHC protocol for PGBD3 antibody 17639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols