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PHC2 Polyclonal antibody

PHC2 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 12867-1-AP

Synonyms

EDR2, HPH2, PH2, PHC2, Polyhomeotic like protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using PHC2 Polyclonal antibody (12867-1-AP)

    WB analysis of A375 using 12867-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12867-1-AP (PHC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PHC2 Polyclonal antibody (12867-1-AP)

    WB analysis of A549 using 12867-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12867-1-AP (PHC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HL-60 cells using PHC2 Polyclonal antibody (12867-1-AP)

    IP experiment of HL-60 using 12867-1-AP

    IP result of anti-PHC2 (IP:12867-1-AP, 4ug; Detection:12867-1-AP 1:500) with HL-60 cells lysate 4000 ug.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using PHC2 Polyclonal antibody (12867-1-AP)

    IF Staining of A375 using 12867-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 12867-1-AP (PHC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA375 cells, A549 cells
Positive IP detected inHL-60 cells
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12867-1-AP targets PHC2 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHC2 fusion protein Ag3507
Full Name polyhomeotic homolog 2 (Drosophila)
Calculated Molecular Weight 858 aa, 91 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC028396
Gene Symbol PHC2
Gene ID (NCBI) 1912
RRIDAB_2877890
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PHC2 antibody 12867-1-APDownload protocol
IF protocol for PHC2 antibody 12867-1-APDownload protocol
IP protocol for PHC2 antibody 12867-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols