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PHYHIPL Polyclonal antibody

PHYHIPL Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16937-1-AP

Synonyms

KIAA1796, PHYHIPL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PHYHIPL Polyclonal antibody (16937-1-AP)

    WB analysis using 16937-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16937-1-AP (PHYHIPL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PHYHIPL Polyclonal antibody (16937-1-AP)

    IHC staining of mouse brain using 16937-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PHYHIPL Polyclonal antibody (16937-1-AP)

    IHC staining of mouse brain using 16937-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PHYHIPL Polyclonal antibody (16937-1-AP)

    IHC staining of mouse testis using 16937-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PHYHIPL Polyclonal antibody (16937-1-AP)

    IHC staining of mouse testis using 16937-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16937-1-AP (PHYHIPL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PHYHIPL Polyclonal antibody (16937-1-AP)

    IF Staining of mouse brain using 16937-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 16937-1-AP (PHYHIPL antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16937-1-AP targets PHYHIPL in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHYHIPL fusion protein Ag10435
Full Name phytanoyl-CoA 2-hydroxylase interacting protein-like
Calculated Molecular Weight 376 aa, 43 kDa
Observed Molecular Weight 39 kDa and 42-45 kDa
GenBank Accession NumberBC011268
Gene Symbol PHYHIPL
Gene ID (NCBI) 84457
RRIDAB_2163761
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHYHIPL, a kind of mitochondrial protein, is associated with energy production, oxidative stress and organelle dynamics. It is reported that global ischemia is related to the change of PHYHIPL protein level. Compared with the control group, the level of APOE (may be involved in abnormal regulation of mitochondrial function) is significantly increased after ischemic stimulation, while the APOE genotype has a effect on PHYHIPL protein level. 16937-1-AP detects 39 kDa isoform and 42-45 kDa isoform in SDS-PAGE. (PMID: 21878944)

Protocols

Product Specific Protocols
WB protocol for PHYHIPL antibody 16937-1-APDownload protocol
IHC protocol for PHYHIPL antibody 16937-1-APDownload protocol
IF protocol for PHYHIPL antibody 16937-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols