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  • Featured Product
  • KD/KO Validated

PIGA Polyclonal antibody

PIGA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13679-1-AP

Synonyms

GlcNAc PI synthesis protein, GPI3, PIG A, PIGA

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PIGA Polyclonal antibody (13679-1-AP)

    WB analysis of HeLa using 13679-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13679-1-AP (PIGA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PIGA Polyclonal antibody (13679-1-AP)

    WB analysis of HEK-293 using 13679-1-AP

    WB result of PIGA antibody (13679-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIGA transfected HEK-293 cells.

  • Western Blot (WB) analysis of HeLa cells using PIGA Polyclonal antibody (13679-1-AP)

    WB analysis of HeLa using 13679-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13679-1-AP (PIGA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using PIGA Polyclonal antibody (13679-1-AP)

    IHC staining of mouse lung using 13679-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using PIGA Polyclonal antibody (13679-1-AP)

    IHC staining of mouse lung using 13679-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using PIGA Polyclonal antibody (13679-1-AP)

    IHC staining of rat liver using 13679-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using PIGA Polyclonal antibody (13679-1-AP)

    IHC staining of rat liver using 13679-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13679-1-AP (PIGA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inmouse lung tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13679-1-AP targets PIGA in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIGA fusion protein Ag4530
Full Name phosphatidylinositol glycan anchor biosynthesis, class A
Calculated Molecular Weight 484 aa, 54 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC038236
Gene Symbol PIGA
Gene ID (NCBI) 5277
RRIDAB_2164656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PIGA antibody 13679-1-APDownload protocol
IHC protocol for PIGA antibody 13679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF,WB

Hum Mol Genet

CNS glycosylphosphatidylinositol deficiency results in delayed white matter development, ataxia and premature death in a novel mouse model.

Authors - Marshall Lukacs
  • KO Validated
View Article