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PKNOX2 Polyclonal antibody

PKNOX2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20352-1-AP

Synonyms

Homeobox protein PKNOX2, Homeobox protein PREP 2, PBX/knotted 1 homeobox 2, PBX/knotted homeobox 2, PKNOX2, PREP2

Validation Data Gallery

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  • Western Blot (WB) analysis of RAW264.7 using PKNOX2 Polyclonal antibody (20352-1-AP)

    WB analysis of RAW264.7 using 20352-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using PKNOX2 Polyclonal antibody (20352-1-AP)

    WB analysis of mouse small intestine using 20352-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PKNOX2 Polyclonal antibody (20352-1-AP)

    WB analysis of HeLa using 20352-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PKNOX2 Polyclonal antibody (20352-1-AP)

    WB analysis of mouse brain using 20352-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using PKNOX2 Polyclonal antibody (20352-1-AP)

    WB analysis of mouse lung using 20352-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using PKNOX2 Polyclonal antibody (20352-1-AP)

    WB analysis of human skeletal muscle using 20352-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20352-1-AP (PKNOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PKNOX2 Polyclonal antibody (20352-1-AP)

    IHC staining of human kidney using 20352-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20352-1-AP (PKNOX2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PKNOX2 Polyclonal antibody (20352-1-AP)

    IHC staining of human kidney using 20352-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20352-1-AP (PKNOX2 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inRAW 264.7 cells, RAW264.7 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20352-1-AP targets PKNOX2 in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKNOX2 fusion protein Ag14195
Full Name PBX/knotted 1 homeobox 2
Calculated Molecular Weight 472 aa, 52 kDa
Observed Molecular Weight 70 kDa, 55 kDa
GenBank Accession NumberBC045626
Gene Symbol PKNOX2
Gene ID (NCBI) 63876
RRIDAB_10694277
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKNOX2, PBX/knotted 1 homeobox 2, also named as PREP2, belongs to the three-amino acid loop extension (TALE) homeobox family. As it contains a DNA-binding motif, PKNOX2 may function as a nuclear transcription factor, experiments have reported the PKNOX2 protein locates to the nucleus. It was showed that highest expression of a 4-kb PREP2 (PKNOX2) transcript in heart, brain, skeletal muscle, and ovary(PMID:11972344). Several years later, PKNOX2 was identified as one of the cis-regulated genes for alcohol addiction in mice, however it has not been reported to be associated with any similar phenotype in humans to date (PMID:19721000). The molecular weight of the protein may differ from the theoretical value.

Protocols

Product Specific Protocols
WB protocol for PKNOX2 antibody 20352-1-APDownload protocol
IHC protocol for PKNOX2 antibody 20352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biochem Biophys Res Commun

PKNOX2 regulates myofibroblast functions and tubular cell survival during kidney fibrosis.

Authors - Yoshiaki Miyake
View Article
pigWB,RIP

Int J Mol Sci

LncRNA TCONS_00323213 Promotes Myogenic Differentiation by Interacting with PKNOX2 to Upregulate MyoG in Porcine Satellite Cells

Authors - Mengxun Li
View Article