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PLAG1 Polyclonal antibody

PLAG1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 18018-1-AP

Synonyms

PLAG1, pleiomorphic adenoma gene 1, PSA, SGPA, Zinc finger protein PLAG1

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using PLAG1 Polyclonal antibody (18018-1-AP)

    WB analysis of A431 using 18018-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 18018-1-AP (PLAG1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PLAG1 Polyclonal antibody (18018-1-AP)

    WB analysis of HepG2 using 18018-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18018-1-AP (PLAG1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PLAG1 Polyclonal antibody (18018-1-AP)

    IP experiment of HepG2 using 18018-1-AP

    IP result of anti-PLAG1 (IP:18018-1-AP, 4ug; Detection:18018-1-AP 1:300) with HepG2 cells lysate 2400ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PLAG1 Polyclonal antibody (18018-1-AP)

    IF Staining of HepG2 using 18018-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18018-1-AP (PLAG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18018-1-AP is PLAG1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLAG1 fusion protein Ag12566
Full Name pleiomorphic adenoma gene 1
Calculated Molecular Weight 500 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC075047
Gene Symbol PLAG1
Gene ID (NCBI) 5324
RRIDAB_2878482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PLAG1 antibody 18018-1-APDownload protocol
IF protocol for PLAG1 antibody 18018-1-APDownload protocol
IP protocol for PLAG1 antibody 18018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols